Bold Line Cage Incubator
Large volume incubator with local CO2/O2 climate chamber
Bold Line Cage Incubator consist of:
• Microscope enclosure
• Obscuring panels (optional)
• Temperature controller (includes air heater)
• Filtering box (optional)
• Stage top gas chamber
• Gas controller (CO2 or dual CO2 /O2 )
• Humidity module
• Touch screen interface
• Smart box (optional)
The Bold Line Cage Incubator controls temperature in the entire enclosure, by receiving feedback from sample temperature. Gas composition and humidity are controlled inside the stage top gas chamber.
Microscope Enclosure
The microscope enclosure creates a large volume around the microscope in which temperature is controlled by recirculating warm air. It is made of polycarbonate panels and encloses microscope stage, nosepiece and objectives. It doesn't affect focus stability.
Features
- • Temperature range: from 3°C above ambient to 45°C
- • Temperature accuracy on sample: 0.1°C
- • Models for any inverted / upright microscope
- • Sliding doors on the front and side panels allow full and easy access to the microscope
- • Removable front panel with turn-to-open hinges
- • Optional black panels for imaging in dark environment- can be added at any time after purchase
- • An LED light with foot pedal and dimmer allows illumination inside the enclosure
- • CO2 (and / or O2) levels set with Digital (Bold Line) or Manual Gas Controllers
- • Humidity: Vibration Free Humidity Module or Active Humidity Controller
Temperature Unit
Okolab Cage Incubator employs a Temperature Unit that generates warm air to raise the temperature inside the enclosure.
Superior accuracy is guaranteed by the interplay of four sensors: three for temperature and one for flow rate. The temperature sensors monitor temperature inside the enclosure, inside the Gas Micro-Environmental Chamber and inside the Air Heater. The flow rate sensor controls the rate at which warm air enters the enclosure and ensures smooth operation and fast heating. A proprietary smart algorithm determines the minimum flow rate necessary to maintain the controlled temperature at the set point value.
Okolab Cage Incubation System has both Enclosure (Chamber) Feedback mode as well as Sample Feedback mode as standard features. The end user can select the temperature sensor driving feedback to the controller.
Features
- • Temperature range: from 3°C above ambient to 45°C
- • Temperature accuracy: 0.1°C
- • # 3 Temperature sensors: in the enclosure; in the gas chamber; inside the air heater
- • # 1 Flow rate sensor inside the air heater
- • Operated via touch screen interface OKO-TOUCH
- • Double air input and output ensures best temperature uniformity inside the enclosure
- • Low energy consumption thanks to warm air recirculation
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Gas Chamber
The micro-environmental Gas Chamber fits on the microscope stage and hosts the sample. The Gas Chamber is located inside the enclosure and at the set point temperature - typically 37°C. The purpose for the Gas Chamber is to create a small volume surrounding the sample and containing humidified gas of the desired concentration.
Features
- • Chambers available for any stage brand/model including Z piezo and upright.
- • Magnetic inserts for Petri Dishes, Slides and Multi Well Plates
- • Magnetic locks hold Petri, Slides and Multi Well Plates in the correct position inside the chamber
- • Built-in Perfusion holes
- • Temperature sensor inlet port for sample feedback mode operation
- • Perfusion holes available for inlet and outlet of tubes
- • Sliding lid for easy access to the sample
- • Dual gas chamber featuring two independent gas compartments for imaging under separate gas environments in the same experiment
CO2 - O2 CONTROLLERS
The Bold Line Gas Controllers connect to the Temperature Controller via a cable-less connector and are operated through the same touch screen interface OKO-TOUCH.
CO2 and/or O2 accuracy is ±0.1% with adjustable total output flow rate.
A vibration free humidity module is typically used with a Cage Incubation System. When Humidity control with feedback is required, the Active Humidity Controller can be connected to the Bold Line Gas Controller.
Features
- • CO2 accuracy: 0.1% (see Controller page for more info)
- • O2 accuracy: 0.1% (see Controller page for more info)
- • Touch Screen Interface - OKO-TOUCH
- • Output Flow Rate: adjustable from OKO-TOUCH
- • Compatible with Active Humidity Controller
- • Programmable CO2 (and /or) O2 cycles
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Vibration Free Humidity Module
The Cage Incubator can be equipped with a vibration-free gas humidifier which employes a semi-permeable membrane. Humidification occurs without bubbling to ensure vibration-free operation.
A water trap is located between the humidifier and the gas chamber preventing any water droplet from accidentally reaching the sample.
Features
- • Gas is humidified through a semi-permeable membrane
- • No bubbling, i.e. no vibrations
- • Fits inside the enclosure
- • Re-filler without opening the enclosure and without perturbing the sample
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OKO-TOUCH
OKO-TOUCH is an intuitive and user-friendly touch screen interface. It connects to any one of the Bold Line Controllers, detects all equipment in the system and displays all functions supported by the equipment detected.
OKO-TOUCH features on-board memory for data logging and mini-USB port for data download.
It allows to:
- • Turn all Bold Line Controllers on/off
- • Define set point values
- • Set flow rates of the Temperature Unit
- • Set flow rates gas controller
- • Define gas cycles of gas controller
- • Select sample or enclosure feedback mode
- • Display all set points and measured parameters
- • Run self calibration procedures
SMART BOX
Smart Box connects to the Bold Line Controller and provides the following features:
Features
- • Data Logging
- • Remote Operation of the incubator via Internets, Tablet or SmartPhone
- • Video Streaming server
- • Remote assistant